Composite

Part:BBa_K593009:Design

Designed by: Ceren Seref   Group: iGEM11_METU-Ankara   (2011-09-21)


ROSE regulated kill switch


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 884
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 884
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 884
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 884
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 884
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1633
    Illegal BsaI.rc site found at 800


Design Notes

No problems occured during assembly process. All parts were compatible with assembly standard 10, so ther weren't any complications about ligating these parts. Also there weren't any problems observed about translation.


Source

All parts that produce that composite part come from 2011 kit plate distribution.

References